Reporter

Part:BBa_K1343014:Design

Designed by: Tal Naor, Ricarina Rabinovitz, Ittai Rubinstein, Yair Maimon   Group: iGEM14_Technion-Israel   (2014-09-27)


Plux-green renilla luciferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 64
    Illegal XhoI site found at 57
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 188
    Illegal AgeI site found at 346
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To assamble this part, and others like it, we used a specialized Gibson Barcode System to allow for a modular construction of gates with various components using the same Gibson reaction conditions, allowing for the assembly of a large number of constructs in parallel to each other


Source

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References